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Home >> Antibodies >> Bromodeoxyuridine (BrdU) (Proliferation Marker)

Bromodeoxyuridine (BrdU) (Proliferation Marker)

Mouse Monoclonal Antibody [Clone BRD469 + BRD494 + BRD.3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD469+BRD494+BRD.3).

Product Details

Synonyms

Bromodeoxyuridine, BUdr

Positive Control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Use Ab at 1ug/ml for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC (Cat. #AP-9008)]Optimal dilution for a specific application should be determined.

Immunogen

Bromodeoxyuridine (BrdU) conjugated to BSA or KLH

Cellular Localization

Nuclear

Species Reactivity

All species

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

Depends on the target

Specificity & Comments

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  2. Williams LS et. al. Cytometry, 1990, 11(4):490-7.

Bioinformatics

NBT01000