Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD469+BRD494+BRD.3).

Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD469+BRD494+BRD.3).

Bromodeoxyuridine (BrdU) (Proliferation Marker) [BRD469 + BRD494 + BRD.3]

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Summary

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

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Product Properties & Targets

Host
Mouse
Species Reactivity
All species
Isotype
IgG1
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
Depends on the target
Immunogen
Bromodeoxyuridine (BrdU) conjugated to BSA or KLH

Database Links

Entrez Gene ID
Not Applicable
Unigene
Not Applicable
SwissProt
N/A

Additional Information

Clone
BRD469 + BRD494 + BRD.3

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Use Ab at 1u�g/ml for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC (Cat. #AP-9008)]Optimal dilution for a specific application should be determined.

Key References

  • Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  • Williams LS et. al. Cytometry, 1990, 11(4):490-7.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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