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Home >> Antibodies >> ATG5 (Autophagy Marker)

ATG5 (Autophagy Marker)

Mouse Monoclonal Antibody [Clone ATG5/2492]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).

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Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).

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Western Blot Analysis of THP1 cell lysate and Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2492).

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SDS-PAGE Analysis Purified ATG5 Mouse Monoclonal Antibody (ATG5/2492). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

APG5 autophagy 5 like (APG5L); Apoptosis-specific protein (ASP); ATG5; Autophagy related 5; hAPG5

Positive Control

THP1, PANC-1, Raji or HeLa cells. Brain, Ovary, Endometrium, Colon or Duodenum (IHC).

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

50-32kDa

Specificity & Comments

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lanza, F., et al. 1991. J. Biol. Chem. 266: 10638-10645.

Bioinformatics

  •  Entrez Gene ID: 9474
  •  Gene Symbol: ATG5
  •  SwissProt: Q9H1Y0
  •  Chromosome Location: 17p11.2
  •  Unigene: 486063
NBT02492