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ATG5 (Autophagy Marker) Antibody [ATG5/2553]

In Stock
Catalog Number Formulation Size Price
9474-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
9474-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
9474-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
ATG5 (Autophagy Marker) Antibody

Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).

ATG5 (Autophagy Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Uterus stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).

ATG5 (Autophagy Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).

ATG5 Antibody in SDS-PAGE

ATG5 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified ATG5 Mouse Monoclonal Antibody (ATG5/2553). Confirmation of Purity and Integrity of Antibody.

ATG5 Antibody in Western Blot (WB)

ATG5 Antibody in Western Blot (WB)

Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver, Guinea pig Liver and Rabbit Liver tissue lysates using ATG5 Mouse Monoclonal Antibody (ATG5/2553).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Preautophagosomal structure membrane
Gene Name
ATG5
Positive Control
A431, Colon or Duodenum., Endometrium, K562, PANC-1, Raji or HeLa cells. Ovary, THP-1, U-87 or Human Liver
Immunogen
Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)
Alternate Names
Autophagy protein 5, APG5-like, Apoptosis-specific protein, APG5 autophagy 5 like (APG5L); Apoptosis-specific protein (ASP); ATG5; Autophagy related 5; hAPG5

Database Links

Entrez Gene ID
9474
SwissProt
Q9H1Y0

Additional Information

Clone
ATG5/2553
Chromosome Location
17p11.2
Mol. Weight of Antigen
32kDa

Related Antibodies

View All ATG5 Antibodies

Functions

  • Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
  • May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
  • (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed:19666601).

Key References

  • Lanza, F., et al. 1991. J. Biol. Chem. 266: 10638-10645.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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