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Home >> Antibodies >> Actin, Smooth Muscle (Leiomyosarcoma Marker)

Actin, Smooth Muscle (Leiomyosarcoma Marker)

Mouse Monoclonal Antibody [Clone ACTA2/791]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).

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Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with Smooth Muscle Actin Monoclonal Antibody (ACTA2/791).

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Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin MAb (ACTA2/791).

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SDS-PAGE Analysis Purified Smooth Muscle Actin MAb (ACTA2/791). Confirmation of Integrity and Purity of Antibody.

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Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin MAb (ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

Product Details

Synonyms

ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Positive Control

Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ACTA2 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Rat. Predicted to show a broad reactivity.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

42kDa

Specificity & Comments

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gown, A.M., et al. 1985. A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes. J. Cell Biol. 100: 807-813.

Bioinformatics

  •  Entrez Gene ID: 59
  •  Gene Symbol: ACTA2
  •  SwissProt: P62736
  •  Chromosome Location: 10q23.31
  •  Unigene: 500483
NBT00791