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TFAM / TCF6 (Transcription factor A, mitochondrial) / mtTFA Antibody [TFAM/9713]

In Stock
Catalog Number Formulation Size Price
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)


mtTFA (mitochondrial transcription factor A), also known as mtTF1, TFAM, TCF6 (for transcription factor 6-like1), TCF6L2 and tsHMG, is a nuclear-encoded gene product that is imported into the mitochondria. mtTFA is required for many aspects of mitochondrial biogenesis including the replication and transcription of mitochondrial DNA (mtDNA). In mouse, testis-specific mtTFA is missing the mitochondria targeting sequence and is present in the nucleus rather than the mitochondria. This form of mtTFA is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. During mouse and human spermatogenesis there is a reduction of mtTFA protein levels and a reduction in mtDNA copy number. These features may provide one of the mechanisms by which paternal mtDNA transmission is prevented. mtTFA has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting.

Product Properties & Targets

Antibody Type
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Mitochondrion, Mitochondrion matrix
Gene Name
Positive Control
Recombinant fragment (around aa1-200) of human TFAM protein (exact sequence is proprietary)
Alternate Names
transcription factor A, mitochondrial, anscription factor 6-like 1 antibody; Mitochondrial transcription factor 1 antibody; mitochondrial transcription factor A antibody; MtTF1 antibody; mtTFA antibody; TCF 6 antibody; TCF-6 antibody; TCF6 antibody; TCF6L1 antibody; TCF6L2 antibody; TCF6L3 antibody; TFAM antibody; TFAM_HUMAN antibody; Transcription factor 6 antibody; Transcription factor 6 like 2 (mitochondrial transcription factor) antibody; Transcription factor 6 like 2 antibody; Transcription factor 6-like 2 antibody; transcription factor 6-like 3 antibody; Transcription factor A, mitochondrial antibody; Transcription factor A, mitochondrial antibody; Transcription factor A, mitochondrial precursor antibody, TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3; MTDPS15

Database Links

Entrez Gene ID

Additional Information

Chromosome Location
Mol. Weight of Antigen


  • Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.

Key References

  • He B, Gao R, Lv S, et al. Cancer cell employs a microenvironmental neural signal trans-activating nucleus-mitochondria coordination to acquire stemness. Signal Transduct Target Ther. 2023;8(1):275. Published 2023 Jul 19. doi:10.1038/s41392-023-01487-4
  • Huang Q, Wu D, Zhao J, et al. TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. EMBO J. 2022;41(11):e110324. doi:10.15252/embj.2021110324
  • Hu M, Zhou M, Bao X, et al. ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage and cGAS/STING activation. J Clin Invest. 2021;131(3):e139333. doi:10.1172/JCI139333
  • Ruan X, Cao M, Yan W, et al. Cancer-cell-secreted extracellular vesicles target p53 to impair mitochondrial function in muscle. EMBO Rep. 2023;24(9):e56464. doi:10.15252/embr.202256464

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.


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