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Cytokeratin 4 (KRT4) Antibody [KRT4/2804]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
3851-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3851-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3851-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Gene Name
Positive Control
A549 or A431 cells. Skin, Cervix or Esophagus., HeLa
Immunogen
Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)
Alternate Names
Keratin, type II cytoskeletal 4, Cytokeratin-4, Keratin-4, Type-II keratin Kb4, CK4; CYK4; Cytokeratin 4; Keratin 4; Keratin type II cytoskeletal 4; KRT4

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
KRT4/2804
Chromosome Location
12q13.13
Mol. Weight of Antigen
~67kDa

Key References

  • Opitz Og, et al. J Biol Chem. 1998 Sep 11;273(37):23912-21.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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