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Printable Datasheet

Cytokeratin, Multi (Epithelial Marker) Antibody [C11]

In Stock
Catalog Number Formulation Size Price
MSM1-393-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM1-393-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM1-393-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

Formalin-fixed, paraffin-embedded Rat Oviduct stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

Formalin-fixed, paraffin-embedded Rat Oviduct stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

SDS-PAGE Analysis of Purified Multi Cytokeratin Mouse Monoclonal Antibody (C11).

SDS-PAGE Analysis of Purified Multi Cytokeratin Mouse Monoclonal Antibody (C11).

Immunofluorescence Analysis of HeLa cells labeling Multi Cytokeratin with Multi Cytokeratin Mouse Monoclonal Antibody (C11) conjugated with CF640R(Green). The nuclear counterstain is DAPI (Blue)

Immunofluorescence Analysis of HeLa cells labeling Multi Cytokeratin with Multi Cytokeratin Mouse Monoclonal Antibody (C11) conjugated with CF640R(Green). The nuclear counterstain is DAPI (Blue)

Flow Cytometric Analysis of Human HeLa cells using Multi Cytokeratin Mouse Monoclonal Antibody (C11) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometric Analysis of Human HeLa cells using Multi Cytokeratin Mouse Monoclonal Antibody (C11) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Frog, Goat, Guinea Pig, Human, Marmoset, Mouse, Pig, Rat
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT4
Positive Control
HeLa or A431 cells. Skin or colon carcinoma.
Immunogen
Keratin-enriched preparation from cultured human A431
Alternate Names
Keratin, type II cytoskeletal 4, Cytokeratin-4, Keratin-4, Type-II keratin Kb4

Database Links

Entrez Gene ID
3851; 3852; 3853; 3856; 3858; 3860; 3875
SwissProt
P19013; P13647; P02538 ; P05787; P13645; P13646; P05783

Additional Information

Clone
C11
Mol. Weight of Antigen
Multiple

Related Antibodies

View All KRT4 Antibodies

Key References

  • Folia Biol., 35(6), 373-382, (1989)
  • Vojtek et al. Neoplasma 37(3), 333-342 (1990).
  • Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).
  • Kovaik et al. Int.J.Cancer, Suppl. 3: 50-55, (1988).
  • Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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