Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

Formalin-fixed, paraffin-embedded Rat Oviduct stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).

SDS-PAGE Analysis Purified Multi Cytokeratin Mouse Monoclonal Antibody (C11).

Immunofluorescence Analysis of HeLa cells labeling Multi Cytokeratin with Multi Cytokeratin Mouse Monoclonal Antibody (C11) conjugated with CF640R(Green). The nuclear counterstain is DAPI (Blue)

Flow Cytometric Analysis of Human HeLa cells using Multi Cytokeratin Mouse Monoclonal Antibody (C11) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Printable Datasheet

Cytokeratin, Multi (Epithelial Marker) [C11]

Name: Cytokeratin, Multi (Epithelial Marker)
SKU: MSM1-393
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Host

Mouse

Species Reactivity

Cow, Frog, Goat, Guinea Pig, Human, Marmoset, Mouse, Pig, Rat

Isotype

IgG1

Gene Name

KRT4

Mol. Weight of Antigen

Multiple

Immunogen

Keratin-enriched preparation from cultured human A431

Database Links

Entrez Gene ID

3851; 3852; 3853; 3856; 3858; 3860; 3875

Unigene

433845; 700779 ; 411501; 99936; P13646; 406013

SwissProt

P19013; P13647; P02538 ; P05787; P13645; P13646; P05783

Additional Information

Clone

C11

Human Gene Symbol

KRT4; KRT5; KRT6; KRT8; KRT10; KRT13; KRT18

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Folia Biol., 35(6), 373-382, (1989)
Vojtek et al. Neoplasma 37(3), 333-342 (1990).
Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).
Kovaik et al. Int.J.Cancer, Suppl. 3: 50-55, (1988).
Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.