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Western Blot Analysis of THP-1, Human Kidney and Human Heart lysates using CD31 Mouse Monoclonal Antibody (PECAM1/3533).
Formalin-fixed, paraffin-embedded human placenta stained with CD31 Mouse Monoclonal Antibody (PECAM1/3533). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human placenta stained with CD31 Mouse Monoclonal Antibody (PECAM1/3533). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31 Mouse Monoclonal Antibody (PECAM1/3533) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein
SDS-PAGE Analysis Purified CD23 Mouse Monoclonal Antibody (CD31/3533). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of Human Heart, Mouse Heart, Rat Heart, Hamster Heart, Guinea pig Heart and Rabbit Heart tissue lysates using CD31 Mouse Monoclonal Antibody (PECAM1/3533).
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
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