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Annexin A1 / (Hairy Cell Leukemia Marker) Antibody [ANXA1/3566]

In Stock
Catalog Number Formulation Size Price
301-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
301-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
301-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

SDS-PAGE Analysis Purified Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence staining of paraformaldehyde-fixed HeLa cells with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).

Immunofluorescence staining of paraformaldehyde-fixed HeLa cells with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).

Flow Cytometric Analysis of PFA-fixed HeLa cells using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of PFA-fixed HeLa cells using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Lymph Node stained withAnnexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Formalin-fixed, paraffin-embedded human Lymph Node stained withAnnexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Formalin-fixed, paraffin-embedded human Lymph Node stained withAnnexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Formalin-fixed, paraffin-embedded human Lymph Node stained withAnnexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, Dendritic Cell Marker, Signal Transduction
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Apical cell membrane, Basolateral cell membrane, Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Early endosome, Endosome membrane, Extracellular exosome, Extracellular space, Lateral cell membrane, Membrane, Nucleus, Phagocytic cup, Secreted, Secretory vesicle lumen
Gene Name
ANXA1
Positive Control
A431 and K562 cell lysates, A549, HeLa, human esophagus, placenta and prostate tissues.
Immunogen
Recombinant full-length human Annexin A1 protein
Alternate Names
Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35, ANXA1; ANX1; Annexin-1; Chromobindin-9; LPC1; p35

Database Links

Entrez Gene ID
301
SwissProt
P04083

Additional Information

Clone
ANXA1/3566
Chromosome Location
9q21.13
Mol. Weight of Antigen
38kDa

Related Antibodies

View All ANXA1 Antibodies

Functions

  • Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).
  • Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:22879591, PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).

Key References

  • Falini B, Tiacci E, Liso A et al. J Exp Med 199:59-68, 2004.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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