Home >> Antibodies >> PU.1 (SPI-1) (B-Cell Marker)

PU.1 (SPI-1) (B-Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rPU1/2146]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

SDS-PAGE Analysis Purified PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146).

SDS-PAGE Analysis Purified PU.1 Recombinant Mouse Monoclonal Antibody (rPU1/2146). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Positive Control

Ramos, K562 or THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 16-170) of human PU.1 protein (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

40kDa

Specificity & Comments

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.