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PU.1 (SPI-1) (B-Cell Marker) Antibody [PU1/2118]

Additional information

Catalog Number: 6688-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6688-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6688-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6688-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PU.1 (SPI-1) (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2118).

PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2118).

PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2118).

PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 4

Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2118).

PU.1 (SPI-1) (B-Cell Marker) Antibody - Image 5

SDS-PAGE Analysis Purified PU.1 Mouse Monoclonal Antibody (PU1/2118). Confirmation of Integrity and Purity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
PU1/2118
Isotype
IgG1
Cellular Localization
Nucleus
Gene Name
SPI1
Positive Control
Colon carcinoma., Hodgkin s Lymphoma, K-562 cells. Lymph node, Spleen
Immunogen
Recombinant human PU.1 fragment around aa 16-170 (Exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).
7
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Additional information

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Reactivity:

, , , ,

Gene:

Host:

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2
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Additional information

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Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Key References
  • Loddenkemper C, et al. J Pathol. 2004; 202:60-9.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6688
SwissProt
P17947
Chromosome Location
11p11.2
Mol. Weight of Antigen
40kDa
Alternate Names
Transcription factor PU.1, 31 kDa-transforming protein, Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Summary

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

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