Home >> Antibodies >> Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker)

Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone PDPN/4009R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human seminoma stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R). Inset: PBS instead of primary antibody; secondary only negative contro

Formalin-fixed, paraffin-embedded human seminoma stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R). Inset: PBS instead of primary antibody; secondary only negative contro

Product Details

Synonyms

Aggrus; Glycoprotein 36 KD; Glycoprotein 36; gp36; GP38; GP40; HT1A1; hT1alpha1; hT1alpha2; Lung type I cell membrane associated glycoprotein; Lung type I cell membrane associated glycoprotein T1A 2; OTS8;

Positive Control

Human tonsil, cervical or lung carcinoma tissues.

Known Applications & Suggested Dilutions

ELISA, IF, IHC-P More Details

ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-100) of human PDPN protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium �as well as lymphoangiomas, �Kaposi sarcomas, �and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.