2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> PIK3R2 / Phosphoinositide-3-kinase regulatory subunit 2

PIK3R2 / Phosphoinositide-3-kinase regulatory subunit 2

Mouse Monoclonal Antibody [Clone PIK3R2/292]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human spleen stained with PIK3R2 Mouse Monoclonal Antibody (PIK3R2/292). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with PIK3R2 Mouse Monoclonal Antibody (PIK3R2/292). Inset: PBS instead of primary antibody; secondary only negative control.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using PIK3R2-Monospecific Mouse Monoclonal Antibody (PIK3R2/292).

Product Details

Synonyms

p85 beta; P85B; Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta); Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta); PI3 kinase p85 beta subunit; PI3K regulatory subunit beta; PIK3R2; PtdIns-3-kinase regulatory subunit p85-beta

Positive Control

Human spleen or bladder.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human PIK3R2 protein (around aa 520-720) (exact sequence is proprietary)

Cellular Localization

Cytoplasm > golgi apparatus. Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

85kDa

Specificity & Comments

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85 and p85), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110 and p110 interact with p85, and p110 has also been shown to interact with p85 in vitro. p110 L expression is restricted to white blood cells. It has been shown to bind p85 and p85, but it apparently does not phosphorylate these subunits. p110 L seems to have the capacity to autophosphorylate. p110 does not interact with the p85 subunits. It has been shown to be activated by and heterotrimeric G proteins.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Vallejo-D az, Jes s et al. Targeted depletion of PIK3R2 induces regression of lung squamous cell carcinoma. Oncotarget vol. 7,51 (2016): 85063-85078. doi:10.18632/oncotarget.13195

Bioinformatics

  •  Entrez Gene ID: 5296
  •  Gene Symbol: PIK3R2
  •  SwissProt: O00459
  •  Chromosome Location: 19p13.11
  •  Unigene: 371344