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Home >> Antibodies >> PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker)

Mouse Monoclonal Antibody [Clone PCNA/6580]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human lymph node stained with PCNA Mouse Monoclonal Antibody (PCNA/6580) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

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Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Mouse Monoclonal Antibody (PCNA/6580) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human kidney cancer stained with PCNA Mouse Monoclonal Antibody (PCNA/6580) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified PCNA Mouse Monoclonal Antibody (PCNA/6580). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of Raji cell lysate using PCNA Mouse Monoclonal Antibody (PCNA/6580).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PCNA Mouse Monoclonal Antibody (PCNA/6580).

Product Details

Synonyms

Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

Positive Control

Tonsil or reactive lymph node. SKBr3, HEK293 or U937 cells.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PCNA protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9.

Bioinformatics