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PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody [SPM350]

Additional information

Catalog Number: 5111-MSM1X
Reactivity:

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Gene:

Host:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
5111-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5111-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5111-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Mouse Monoclonal Antibody (SPM350).

PCNA Antibody in SDS-PAGE

PCNA Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Proliferating cell nuclear antigen Mouse Monoclonal Antibody (SPM350). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Chicken, Drosophila melanogaster, Human, Monkey, Mouse, Pig, Pombe, Rat, Yeast (S. pombe and S. cerevisiae), Zebrafish
Clone
SPM350
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
PCNA
Positive Control
Tonsil or reactive lymph node.
Immunogen
Rat PCNA/protein A fusion protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Key References
  • Waseem NH Lane DP. 1990. J Cell Sci. 96:121-9.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5111
SwissProt
P12004
Chromosome Location
20p12.3
Mol. Weight of Antigen
36kDa
Alternate Names
Proliferating cell nuclear antigen, Cyclin, Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

Summary

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

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