Home >> Antibodies >> p40 (Squamous, Basal & Myoepithelial Cell Marker)

p40 (Squamous, Basal & Myoepithelial Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone P40/4396R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Flow cytometric analysis of PFA-fixed HeLa cells. p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Flow cytometric analysis of PFA-fixed HeLa cells. p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Amplified in squamous cell carcinoma (AIS); Chronic ulcerative stomatitis protein (CUSP); EEC3; TP73L; LMS; TP63; Keratinocyte transcription factor KET; NBP; P51/P63; p53 like transcription factor; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; Transformation-related protein 63; Trp53rp1; Trp6;3; Tumor protein p53-like; tumor protein p73-like

Positive Control

HEK293, MDA-MB453 or MCF7 cells. Human prostate, lung or bladder squamous cell carcinoma tissue.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IHC-P More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-100) of human p40 protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

63kDa

Specificity & Comments

p40 antibody (antibody recognizing Np63 only) is an isoform of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.