Formalin-fixed, paraffin-embedded human skin stained with p40 Recombinant Rabbit Monoclonal Antibody (ZR8).

Immunofluorescence Analysis of PFA-fixed HeLa cells labeling p40 Recombinant Rabbit Monoclonal Antibody (ZR8) followed by goat anti-rabbit IgG-CF488 (green); phalloidin counterstain (red).

Flow Cytometric Analysis of PFA-fixed HeLa cells using p40 Recombinant Rabbit Monoclonal Antibody (ZR8) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human skin stained with p40 Recombinant Rabbit Monoclonal Antibody (ZR8).

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) [ZR8]

Name: p40 (deltaNp63) (Squamous, Basal &amp; Myoepithelial Cell Marker)
SKU: 8626-RBM12
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

p63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) antibody recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Nucleus

Gene Name

TP63

Mol. Weight of Antigen

40kDa

Immunogen

Synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p63 protein

Database Links

Entrez Gene ID

8626

Unigene

137569

SwissProt

Q9H3D4

Additional Information

Clone

ZR8

Human Gene Symbol

TP63

Chromosome Location

3q28

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Signoretti S, et al. Am J Pathol. 2000; 157(6):1769-75.
Terry J, et al. Am J Surg Pathol. 2010; 34(12):1805-11.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.