Home >> Antibodies >> Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1)

Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1)

Rat Monoclonal Antibody [Clone OSP/4589]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human brain stained with Osteopontin Rat Monoclonal Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin Rat Monoclonal Antibody (OSP/4589) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human brain stained with Osteopontin Rat Monoclonal Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin Rat Monoclonal Antibody (OSP/4589) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

BNSP; Bone sialoprotein 1 (BSPI/BSP1); Early T lymphocyte activation 1 (ETA1); Nephropontin; immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); CALPHA1 fusion; Urinary stone protein; Uropontin

Positive Control

Bone. Found in plasma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Functional Studies
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length rat Spp1 protein

Cellular Localization

Secreted.

Species Reactivity

Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.

Host / Ig Isotype

Rat / IgG1

Mol. Weight of Antigen

34.9kDa

Specificity & Comments

Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.