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Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1) Antibody [OSP/4589]

In Stock
Catalog Number Formulation Size Price
6696-RTM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6696-RTM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6696-RTM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human brain stained with Osteopontin Rat Monoclonal Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human brain stained with Osteopontin Rat Monoclonal Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin Rat Monoclonal Antibody (OSP/4589) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin Rat Monoclonal Antibody (OSP/4589) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

Product Properties & Targets

Antibody Type
Rat Monoclonals
Host
Rat
Applications
Flow Cytometry, Functional Studies, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Dog, Human, Mink, Mouse, Rabbit, Rat, Turkey
Research Areas
Cardiovascular, Complement System, Mesenchymal Stem Cell Differentiation, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 /
Cellular Localization
Secreted
Gene Name
SPP1
Positive Control
Bone. Found in plasma.
Immunogen
Recombinant full-length rat Spp1 protein
Alternate Names
Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, BNSP; Bone sialoprotein 1 (BSPI/BSP1); Early T lymphocyte activation 1 (ETA1); Nephropontin; immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); CALPHA1 fusion; Urinary stone protein; Uropontin

Database Links

Entrez Gene ID
6696
SwissProt
P10451

Additional Information

Clone
OSP/4589
Chromosome Location
4q22.1
Mol. Weight of Antigen
34.9kDa

Related Antibodies

View All SPP1 Antibodies

Functions

  • Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
  • Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Key References

  • Solursh M. The Journal of biological chemistry 265.25 (1990 Sep 5): 14956-63.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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