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Home >> Antibodies >> MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

Mouse Monoclonal Antibody [Clone MUC1/520]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/520).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/520).

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Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC-1 / CA15-3 / EMA Mouse Monoclonal Antibody (MUC1/520).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Mouse Monoclonal Antibody (MUC1/520)

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Mouse Monoclonal Antibody (MUC1/520).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Mouse Monoclonal Antibody (MUC1/520).

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SDS-PAGE Analysis Purified MUC-1 / EMA Mouse Monoclonal Antibody (MUC1/520). Confirmation of Integrity and Purity of Antibody

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Western Blot Analysis of human MCF-7 cell lysate using MUC-1 / EMA Mouse Monoclonal Antibody (MUC1/520).

Product Details

Synonyms

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

Positive Control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human milk-fat globule membranes (HMFGM)

Cellular Localization

Cytoplasmic & Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, lambda

Mol. Weight of Antigen

265-400kDa

Specificity & Comments

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Stanley CM, Phillips TE. Am J Physiol. 1999;277:G191-200.

Bioinformatics

  •  Entrez Gene ID: 4582
  •  Gene Symbol: MUC1
  •  SwissProt: P15941
  •  Chromosome Location: 1q22
  •  Unigene: 89603
NBT00520