Contact Us

Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [rMUC1/4418]

Additional information

Catalog Number: 4582-MSM28
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
4582-MSM28-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4582-MSM28-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4582-MSM28-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 / EMA Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified MUC-1 Recombinant Mouse Monoclonal Antibody (rMUC1/4418). Confirmation of Integrity and Purity of Antibody.

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney and Human Lung tissue lysates using MUC-1 Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of Kidney and Lung tissue lysates of different species using MUC-1 Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rMUC1/4418
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Colon, MCF-7 or MDA-231 cells. Breast, ovarian or endometrial carcinoma. Kidney, Lung.
Immunogen
Recombinant full-length human MUC1 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in the CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

SDS-PAGE Analysis of Purified P-Cadherin Mouse Monoclonal Antibody (6A9). Confirmation of Integrity and Purity of Antibody.
0
  • Flow
  • IF
  • WB

P-Cadherin (CDH3) Antibody

Additional information

Catalog Number: 1001-MSM2
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2183).
4
  • IHC
  • WB

Occludin (OCLN) (Tight Junctions Marker) Antibody

Additional information

Catalog Number: 100506658-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).
4
  • IHC
  • WB

Occludin (Marker of Early Blood Brain Barrier Damage) Antibody

Additional information

Catalog Number: 100506658-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded mouse heart stained with N-Cadherin Mouse Monoclonal Antibody (8C11).
3
  • Flow
  • IF
  • IHC
  • WB

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM2
Reactivity:

,

Gene:

Host:

SDS-PAGE Analysis of Purified N-Cadherin Mouse Monoclonal Antibody (13A9). Confirmation of Integrity and Purity of Antibody.
1
  • Flow
  • IF
  • WB

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM1
Reactivity:

,

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM2
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).
7
  • ELISA
  • Flow
  • IHC
  • WB

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM1
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH16/1532R)
6
  • IHC
  • WB
  • Flow

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBM2
Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
  • The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Key References
  • Cordell J et al. 1985. Br J Cancer 52(3):347-54.
  • Heyderman E et al. 1985. Br J Cancer 52(3):355-61.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4582
SwissProt
P15941
Chromosome Location
1q22
Mol. Weight of Antigen
265-400kDa
Alternate Names
Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

Summary

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us