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Home >> Antibodies >> MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone MUC1/2818R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Stomach Carcinoma stained with MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/2818R).

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Formalin-fixed, paraffin-embedded human Colon stained with MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/2818R).

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SDS-PAGE Analysis Purified MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/2818R). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of MCF-7 cell lysate using MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/2818R).

Product Details

Synonyms

Breast carcinoma-associated antigen DF3, CA15-3, H23AG, KL-6, MAM6, Mucin 1 cell surface associated, , Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen

Positive Control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Applications & Suggested Dilutions

Western Blot, ELISA, IHC-P         More Details

Western Blot (1-2ug/ml)
ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA) (Very good capturing Ab)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human milk-fat globule membranes (HMFGM)

Cellular Localization

Cytoplasmic and cell surface

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

265-400kDa

Specificity & Comments

This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Stanley CM, Phillips TE. Am J Physiol. 1999;277:G191-200.

Bioinformatics

  •  Entrez Gene ID: 4582
  •  Gene Symbol: MUC1
  •  SwissProt: P15941
  •  Chromosome Location: 1q22
  •  Unigene: 89603
NBT02818