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Home >> Antibodies >> Mitochondria (Marker for Human Cells)

Mitochondria (Marker for Human Cells)

Mouse Monoclonal Antibody [Clone AE-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with Mitochondria Mouse Monoclonal Antibody (AE-1).

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Western Blot Analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (AE-1).

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Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (AE-1).

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Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (AE-1) Green (CF488) and Reddot is used to label the nuclei Red.

Product Details

Synonyms

Not Known

Positive Control

HeLa or HepG2 cells. Hepatic carcinoma.

Known Applications & Suggested Dilutions

ELISA, IF, Western Blot, IHC-P         More Details

ELISA (For coating, order Ab without BSA)
Immunofluorescence (1-2ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Semi-Purified mitochondrial preparation

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

MAb MTC02 recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC02 recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MAb MTC02 is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Bioinformatics

NBT00905