Mitochondria (Marker for Human Cells) [AE-1]

Clear

Summary

MAb MTC02 recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC02 recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MAb MTC02 is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
60kDa
Immunogen
Semi-purified mitochondrial preparation

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
AE-1
Human Gene Symbol
Not Known
Chromosome Location
Not Known

Known Applications & Suggested Dilutions

  • ELISA (For coating, order Ab without BSA)
  • Immunofluorescence (0.5-1ug/ml)
  • Western Blot (0.25-0.5ug/ml)
  • Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies