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Home >> Antibodies >> Interleukin-7 (IL-7)

Interleukin-7 (IL-7)

Mouse Monoclonal Antibody [Clone IL7/4012]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human adrenal gland stained with Interleukin-7 (IL-7) Mouse Monoclonal Antibody (IL7/4012). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified IL-7Mouse Monoclonal Antibody (IL7/4012). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed U-87 cells stained using Interleukin-7 (IL-7) Mouse Monoclonal Antibody (IL7/4012) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Flow cytometric analysis of PFA-fixed U-87 cells. Interleukin-7 (IL-7) Mouse Monoclonal Antibody (IL7/4012) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing >19,000 full-length human proteins using Interleukin-7 (IL-7) Monospecific Mouse Monoclonal Antibody (IL7/4012).

Product Details

Synonyms

IL 7; IL-7; Il7; Interleukin 7; Interleukin-7; LP-1; Lymphopoietin 1

Positive Control

Human thymus or tonsil tissue.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 27-177) corresponding to mature protein of human IL7(exact sequence is proprietary)

Cellular Localization

Secreted.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

25kDa

Specificity & Comments

Interleukin-7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor specific chain and the IL-2 receptor chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of Insulin receptor substrate-1 (IRS-1) and Insulin receptor substrate-2 (IRS-2), originally called 4PS.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Strauchen JA, Breakstone BA. Am J Pathol. 1987;126(3):506 512.Reche PA, et al. Journal of Immunology. 2001;167(1):336 343. doi:10.4049/jimmunol.167.1.336

Bioinformatics

  •  Entrez Gene ID: 3574
  •  Gene Symbol: IL7
  •  SwissProt: IDP13232
  •  Chromosome Location: 8q12-q13
  •  Unigene: 591873