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IDH1 (Isocitrate Dehydrogenase) Antibody [IDH1/1152]

In Stock
Catalog Number Formulation Size Price
3417-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3417-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3417-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).

Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. IDH1 Mouse Monoclonal Antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.

Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. IDH1 Mouse Monoclonal Antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming 伪-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of 伪-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Infectious Disease, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytosol, Peroxisome
Gene Name
IDH1
Positive Control
colon or prostate carcinoma., HeLa, HePG2, HT29 or MCF7 cells. Human breast
Immunogen
Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)
Alternate Names
Isocitrate dehydrogenase [NADP] cytoplasmic, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD

Database Links

Entrez Gene ID
3417
SwissProt
O75874

Additional Information

Clone
IDH1/1152
Chromosome Location
2q33.3
Mol. Weight of Antigen
45-47kDa

Related Antibodies

View All IDH1 Antibodies

Functions

  • Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

Key References

  • Geisbrecht, B.V. and Gould, S.J. 1999. J. Biol. Chem. 274: 30527-30533.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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