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Recombinant IDH1-R132H (Isocitrate Dehydrogenase) Antibody [IDH1/7277R]

Additional information

Catalog Number: 3417-RBM7
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3417-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3417-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3417-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant IDH1-R132H (Isocitrate Dehydrogenase) Antibody

FFPE human prostate with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit Monoclonal Antibody (IDH1/7277R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
IDH1/7277R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytosol, Peroxisome
Gene Name
IDH1
Positive Control
Human breast, colon or prostate carcinoma. HeLa, HePG2, HT29 or MCF7 cells.
Immunogen
Recombinant fragment of human IDH1 (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Key References
  • Capper D, et al: Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol 2010 Jan;20(1):245-254
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3417
SwissProt
O75874
Chromosome Location
2q33.3
Mol. Weight of Antigen
45-47kDa
Alternate Names
Isocitrate dehydrogenase [NADP] cytoplasmic, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD

Summary

IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.

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