FFPE human glioblastoma with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit Monoclonal Antibody (IDH1/6806R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

FFPE prostate carcinoma with IDH1-R132H mutation stained with IDH1-R132H Recombinant Rabbit Monoclonal Antibody (IDH1/6806R).

IHC analysis of formalin-fixed, paraffin-embedded human cerebellum. Negative tissue control using IDH1/6806R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Printable Datasheet

IDH1-R132H (Isocitrate Dehydrogenase) [IDH1/6806R]

Name: IDH1-R132H (Isocitrate Dehydrogenase)
SKU: 3417-RBM6
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cytoplasm, Cytosol, Peroxisome

Gene Name

IDH1

Mol. Weight of Antigen

45-47kDa

Immunogen

Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)

Database Links

Entrez Gene ID

3417

Unigene

593422

SwissProt

O75874

Additional Information

Clone

IDH1/6806R

Human Gene Symbol

IDH1

Chromosome Location

2q33.3

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Capper D, et al: Characterization of R132H mutation-specific IDH1 antibody binding in brain tumors. Brain Pathol 2010 Jan;20(1):245-254

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.