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Home >> Antibodies >> Golgi Complex (Marker for Human Cells)

Golgi Complex (Marker for Human Cells)

Mouse Monoclonal Antibody [Clone SPM581]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Gallbladder stained with Golgi Monoclonal Antibody (SPM581).

Product Details

Synonyms

Not Known

Positive Control

Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, ICC, IHC-P         More Details

Flow Cytometry (0.5-1.0ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunocytochemistry (Acetone or paraformaldehyde fixed) (1-2ug/ml for 30 min)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

SU-DHL-1 large cell lymphoma cells.

Cellular Localization

Golgi complex in cytoplasm

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Not Known

Specificity & Comments

This MAb recognizes Golgi complex in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex of cells in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Yoshio Endo et. al. Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides. Cancer science 98;2007.
  2. Yuasa, K et. al. Binding and Phosphorylation of a Novel Male Germ Cell-specific cGMP-dependent Protein Kinase-anchoring Protein by cGMP-dependent Protein Kinase I. J Biol Chem, 275(7):4897-4905;2000

Bioinformatics

NBT00102