Formalin fixed paraffin embedded human breast carcinoma stained with ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/2299R).
SDS-PAGE Analysis Purified ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/2299R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor, alpha Rabbit Recombinant MAb (ESR1/2299R).
Flow cytometric analysis of PFA-fixed MCF-7 cells. Estrogen Receptor, alpha Rabbit Recombinant MAb (ESR1/2299R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This MAb is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.