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Home >> Antibodies >> Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Estrogen Receptor, alpha (Marker of Estrogen Dependence)

Recombinant Rabbit Monoclonal Antibody [Clone ESR1/2299R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin fixed paraffin embedded human breast carcinoma stained with ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/2299R).

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SDS-PAGE Analysis Purified ER, alpha Rabbit Recombinant Monoclonal Antibody (ESR1/2299R). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human MCF-7 cell lysate using Estrogen Receptor, alpha Rabbit Recombinant MAb (ESR1/2299R).

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Flow cytometric analysis of PFA-fixed MCF-7 cells. Estrogen Receptor, alpha Rabbit Recombinant MAb (ESR1/2299R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1

Positive Control

MCF-7 cells. Human breast or endometrial carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Estrogen Receptor alpha protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

~67kDa

Specificity & Comments

This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This MAb is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.

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200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zafrani B, et. al. Histopathology 2000; 37(6), 536 545.
  2. Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 1474 1481.

Bioinformatics

  •  Entrez Gene ID: 2099
  •  Gene Symbol: ESR1
  •  SwissProt: P03372
  •  Chromosome Location: 6q25.1
  •  Unigene: 208124