Estrogen Receptor, alpha (Marker of Estrogen Dependence) [ESR1/6983R]

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Summary

Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems. Studies have shown ER 伪 is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas. Studies with immunohistochemical assay show that positive steroid hormone status has predicted favorable overall, survival, independently of hormonal treatment. Secondly, ER 伪 can be used as a tumor marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.

Product Properties & Targets

Host
Species Reactivity
Isotype
IgG
Cellular Localization
Gene Name
ESR1
Mol. Weight of Antigen
~67kDa
Immunogen
Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
ESR1/6983R
Human Gene Symbol
ESR1
Chromosome Location
6q25.1

Known Applications & Suggested Dilutions

  • Western Blot (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Zafrani B, et. al. Histopathology 2000; 37(6), 536 545.
  • Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 1474 1481.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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