Home >> Antibodies >> ER-beta (Estrogen Receptor beta-5)

ER-beta (Estrogen Receptor beta-5)

Mouse Monoclonal Antibody [Clone PPG5/25]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified ER-beta5 Mouse Monoclonal Antibody (PPG5/25). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human breast stained with ER-beta5 Mouse Monoclonal Antibody (PPG5/25).

SDS-PAGE Analysis Purified ER-beta5 Mouse Monoclonal Antibody (PPG5/25). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human breast stained with ER-beta5 Mouse Monoclonal Antibody (PPG5/25).

Product Details

Synonyms

Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2); Oestrogen Receptor Beta; Estrogen Receptor Beta; ER-BETA; ESTRB

Positive Control

HCT cells. Normal liver, colon or colon carcinoma.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P More Details

Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Tuberculin conjugated synthetic peptide (LLSHVRHARYAP); derived from the C-terminus of human Estrogen Receptor beta 5

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

59kDa

Specificity & Comments

This MAb specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). No cross-reactivity is seen with estrogen receptor beta 1 or 2 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.