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ER-beta (Estrogen Receptor beta-2) Antibody [57/3]

In Stock
Catalog Number Formulation Size Price
2100-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2100-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2100-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
MCF-7 cells. Human testis tissue.
Immunogen
A synthetic peptide (CGMKMETLLPEATMEQ ) from the C terminus of the human estrogen receptor beta 2 isomer
Alternate Names
Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2); Oestrogen Receptor Beta; Estrogen Receptor Beta; ER-BETA; ESTRB

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
57/3
Chromosome Location
14q23.2
Mol. Weight of Antigen
59kDa

Functions

  • Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).
  • Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Key References

  • Collins, F., et al. (2009). Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα. BMC Cancer, 9, 330.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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