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ER-beta (Estrogen Receptor ß5) Antibody [PPG5/25]

In Stock
Catalog Number Formulation Size Price
2100-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
2100-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
2100-MSM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
ER-beta (Estrogen Receptor ß5) Antibody

SDS-PAGE Analysis of Purified ER-beta5 Mouse Monoclonal Antibody (PPG5/25). Confirmation of Integrity and Purity of Antibody.

ER-beta (Estrogen Receptor ß5) Antibody - Image 2

Formalin-fixed, paraffin-embedded human breast stained with ER-beta5 Mouse Monoclonal Antibody (PPG5/25).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). No cross-reactivity is seen with estrogen receptor beta 1 or 2 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, Infectious Disease, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
ESR2
Positive Control
colon or colon carcinoma., HCT cells. Normal liver
Immunogen
Tuberculin conjugated synthetic peptide (LLSHVRHARYAP); derived from the C-terminus of human Estrogen Receptor beta 5
Alternate Names
Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2); Oestrogen Receptor Beta; Estrogen Receptor Beta; ER-BETA; ESTRB

Database Links

Entrez Gene ID
2100
SwissProt
Q92731

Additional Information

Clone
PPG5/25
Chromosome Location
14q23.2
Mol. Weight of Antigen
59kDa

Related Antibodies

View All ESR2 Antibodies

Functions

  • Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).
  • Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Key References

  • Collins, F., et al. (2009). Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα. BMC Cancer, 9, 330.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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