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ER-beta (Estrogen Receptor beta-1) Antibody [PPG5/10]

In Stock
Catalog Number Formulation Size Price
2100-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2100-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2100-MSM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified ER-beta1 Mouse Monoclonal Antibody (PPG5/10). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified ER-beta1 Mouse Monoclonal Antibody (PPG5/10). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (PPG5/10).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (PPG5/10).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta1 Mouse Monoclonal Antibody (PPG5/10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta1 Mouse Monoclonal Antibody (PPG5/10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Horse, Human, Monkey, Mouse, Pig, Rat, Sheep
Research Areas
Breast Cancer, Cardiovascular, Infectious Disease, Nuclear Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Nucleus
Gene Name
ESR2
Positive Control
Bladder, Breast, Gastric or Salivary carcinoma, MCF-7 cells. Ovarian
Immunogen
A Synthetic peptide (CEDSKSKEGSQNPQSQ) from the C terminus of the human estrogen receptor beta 1 isomer
Alternate Names
Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2); Oestrogen Receptor Beta; ESTRB

Database Links

Entrez Gene ID
2100
SwissProt
Q92731

Additional Information

Clone
PPG5/10
Chromosome Location
14q23.2
Mol. Weight of Antigen
59kDa

Related Antibodies

View All ESR2 Antibodies

Functions

  • Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).
  • Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Key References

  • Collins, F., et al. (2009). Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα. BMC Cancer, 9, 330.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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