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ER-beta � (Estrogen Receptor beta-1)

Mouse Monoclonal Antibody [Clone PGP5/10]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified ER-beta1 Mouse Monoclonal Antibody (PPG5/10). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (PPG5/10).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta1 Mouse Monoclonal Antibody (PPG5/10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SDS-PAGE Analysis Purified ER-beta1 Mouse Monoclonal Antibody (PPG5/10). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (PPG5/10).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta1 Mouse Monoclonal Antibody (PPG5/10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Synonyms

Estrogen Receptor 2; Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2); Oestrogen Receptor Beta; ESTRB

Positive Control

MCF-7 cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (2-4ug/million cells)
Immunofluorescence (2-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A Synthetic peptide (CEDSKSKEGSQNPQSQ) from the C terminus of the human estrogen receptor beta 1 isomer

Cellular Localization

Nuclear

Species Reactivity

Human, Monkey, Mouse, Rat, Pig, Horse and Sheep.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

59kDa

Specificity & Comments

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.