SDS-PAGE Analysis Purified ER-beta1 Mouse Monoclonal Antibody (PPG5/10). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER-beta1 Mouse Monoclonal Antibody (PPG5/10).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta1 Mouse Monoclonal Antibody (PPG5/10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.