Formalin-fixed, paraffin-embeddedhumanprostate carcinoma stained with EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/826). Inset: PBS instead of primary antibody, secondary negative control.
Formalin-fixed, paraffin-embeddedhumanprostatecarcinoma stained with EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/826).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.