Formalin-fixed, paraffin-embedded human colon stained with EpCAM / CD326 Recombinant Mouse Monoclonal Antibody (rEGP40/7133). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM / CD326 Recombinant Mouse Monoclonal Antibody (rEGP40/7133). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.