Home >> Antibodies >> EpCAM / CD326 (Epithelial Marker)

EpCAM / CD326 (Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone EGP40/4546R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/4546R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/4546R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using EGP40/4546R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/4546R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/4546R).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/4546R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using EGP40/4546R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/4546R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Positive Control

HT29 cells. Human breast tumor.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Ep-CAM protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Does not react with Dog or Cat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

40-43kDa

Specificity & Comments

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.