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Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)

Mouse Monoclonal Antibody [Clone EGP40/1372]

Catalog #


0.5 ml at 100ug/ml

Price (USD)



Purified Ab conjugated to Horse Radish Peroxidase (HRP)

Catalog No


0.5 ml at 100ug/ml


Purified Ab conjugated to Horse Radish Peroxidase (HRP)

Price (USD)


Formalin-fixed, paraffin-embedded Human Ovarian Carcinoma stained with EpCAM Mouse Monoclonal Antibody (EGP40/1372) conjugated to HRP.


Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1372).

Product Details


Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Positive Control

HT29 cells or Ovarian carcinoma

Known Applications & Suggested Dilutions

Flow Cyt, IF, WB, Immunohistology         More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.


Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen


Specificity & Comments

This Primary antibody is directly conjugated to Horse Radish Peroxidase (HRP) enzyme. Compared to indirect-primary-secondary detection system, it offers the following advantages: 1) Assay time is shorter as only one antibody is involved, 2) Background is reduced as there is no secondary antibody, and 3) It can be used to detect the target protein in the antibody-host samples (i.e. Mouse-on-Mouse or Rabbit-on-Rabbit), avoiding problem related to host-immunoglobulins.Epitope of this MAb is mapped between aa 202-209 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Related Products

Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% ProClin 300. Contact us if you require this Ab in a different format.

Key References

  1. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.


  •  Entrez Gene ID: 4072
  •  Gene Symbol: TACSTD1
  •  SwissProt: P16422
  •  Chromosome Location: 2p21
  •  Unigene: 542050