SDS-PAGE Analysis Purified DBC2 Mouse Monoclonal Antibody (DBC2/3361). Confirmation of Purity and Integrity of Antibody.
IHC analysis of formalin-fixed, paraffin-embedded human renal oncocytoma. Staining using DBC2/3361 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Staining using DBC2/3361 at 2ug/ml in PBS for 30min RT.
Analysis of Protein Array containing more than 19,000 full-length human proteins using DBC2 Mouse Monoclonal Antibody (DBC2/3361).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. DBC-2 (deleted in breast cancer 2 gene protein), also known as RHOBTB2 (Rho-related BTB domain-containing protein 2), is a 727 amino acid member of the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. Expressed ubiquitously with highest levels in neural tissue, heart, brain and fetal lung, DBC-2 contains two BTB (POZ) domains through which it may bind to and regulate the function of target proteins, such as CUL-3. Additionally, DBC-2 is thought to function as a regulator of cell cycle and apoptosis events. Under normal conditions, DBC-2 is thought to exhibit tumor suppressor activity. Mutations in the gene encoding DBC-2 are associated with breast cancer, suggesting that mutated DBC-2 may play a role in carcinogenesis.