Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

DBC2 / RHOBTB2 Antibody [DBC2/3364]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
23221-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
23221-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
23221-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. DBC-2 (deleted in breast cancer 2 gene protein), also known as RHOBTB2 (Rho-related BTB domain-containing protein 2), is a 727 amino acid member of the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. Expressed ubiquitously with highest levels in neural tissue, heart, brain and fetal lung, DBC-2 contains two BTB (POZ) domains through which it may bind to and regulate the function of target proteins, such as CUL-3. Additionally, DBC-2 is thought to function as a regulator of cell cycle and apoptosis events. Under normal conditions, DBC-2 is thought to exhibit tumor suppressor activity. Mutations in the gene encoding DBC-2 are associated with breast cancer, suggesting that mutated DBC-2 may play a role in carcinogenesis.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2c / Kappa
Gene Name
Positive Control
Human heart muscle or brain tissue.
Immunogen
Human recombinant RHOBTB2 protein fragment (around aa 554-604) (exact sequence is proprietary)
Alternate Names
Rho-related BTB domain-containing protein 2, Deleted in breast cancer 2 gene protein, p83, Deleted in breast cancer 2 gene protein; p83; Rho related BTB domain containing protein 2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
DBC2/3364
Chromosome Location
8p21.3
Mol. Weight of Antigen
83kDa

Key References

  • Ramos, S., et al. 2002. Gene 298: 147-157.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “DBC2 / RHOBTB2 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK