Home >> Antibodies >> Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker)

Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone KRT5/4245R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (KRT5/4245R).

Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (KRT5/4245R).

Formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (KRT5/4245R).

Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody (KRT5/4245R).

Product Details

Synonyms

58kDa Cytokeratin; DDD1; Epidermolysis Bullosa Simplex 2 (EBS2); Keratin 5; Keratin, Type II Cytoskeletal 5; Keratin-5; Type-II Cytoskeletal 5; Type-II keratin Kb5

Positive Control

MCF-7 or HeLa cells. Human tonsil, esophagus or bladder tissue (IHC).

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa490-590) corresponding to C-terminal of human cytokeratin 5

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

58kDa

Specificity & Comments

This MAb recognizes a protein of 58kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.