Cytokeratin 5/8 [SPM268]



It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Product Properties & Targets

Species Reactivity
Gene Name
Mol. Weight of Antigen
58kDa (CK5); 52.5kDa (CK8)
Cytoskeletal preparation from HeLa cells

Database Links

Entrez Gene ID

Additional Information

Human Gene Symbol
Chromosome Location
12q13.13 (CK5) & 17q21.2 (CK8)

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Key References

  • Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  • Vojtek et al. Folia Biol. 37(3-4): 197-206 (1991).
  • Bartek et al. J. of Pathology 164, 215-24 (1991).
  • Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).

PubMed Links

Storage & Stability

Store at 2 to 8°C. Antibody is stable for 24 months.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

0.5 ml at 200 �g/ml of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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