Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (SPM268).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (SPM268).

Cytokeratin 5/8 [SPM268]

Clear

Summary

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Printable

Product Properties & Targets

Host
Mouse
Species Reactivity
Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep
Isotype
IgG1
Gene Name
KRT5
Mol. Weight of Antigen
58kDa (CK5); 52.5kDa (CK8)
Immunogen
Cytoskeletal preparation from HeLa cells

Database Links

Entrez Gene ID
3852; 3856
Unigene
433845 ; 533782; 708445
SwissProt
P13647 ; P05787

Additional Information

Clone
SPM268
Human Gene Symbol
KRT5 & KRT8
Chromosome Location
12q13.13 (CK5) & 17q21.2 (CK8)

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Key References

  • Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  • Vojtek et al. Folia Biol. 37(3-4): 197-206 (1991).
  • Bartek et al. J. of Pathology 164, 215-24 (1991).
  • Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).

PubMed Links

Storage & Stability

Store at 2 to 8°C. Antibody is stable for 24 months.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

0.5 ml at 200 �g/ml of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Related products

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies

Company Info
Antibodies
View All
Hosts
View All
Isotype
View All
Newsletter Signup NeoBiotechnologies Updates & Sales

$0.00
510-376-5603