Cytokeratin 5/6 (Basal, Myoepithelial & Mesothelial Cell Marker) [KRT5.6/2438]

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Summary

It recognizes polypeptides of 58kDa and 56kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma.

Product Properties & Targets

Host
Species Reactivity
Isotype
Gene Name
KRT5
Mol. Weight of Antigen
58kDa (KRT5); 56kDa (KRT6A)
Immunogen
Recombinant full-length human KRT5 protein

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
KRT5.6/2438
Human Gene Symbol
KRT5 & KRT6
Chromosome Location
12q13.13 (KRT5 and KRT6A);

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Ordonez NG1. Value of cytokeratin 5/6 immunostaining in distinguishing epithelial mesothelioma of the pleura from lung adenocarcinoma. Am J Surg Pathol. 1998; 22:1215-1221.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8锟�C. Antibody without azide - store at -20 to -80锟�C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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