SDS-PAGE Analysis Purified Cytokeratin 5/8 Monoclonal Antibody (C-50). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified Cytokeratin 5/8 Monoclonal Antibody (C-50). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (C-50).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (C-50).

Cytokeratin 5/8 [C-50]

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Summary

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

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Product Properties & Targets

Host
Mouse
Species Reactivity
Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep
Isotype
IgG1
Gene Name
KRT5
Mol. Weight of Antigen
58kDa (CK5); 52.5kDa (CK8)
Immunogen
Cytoskeletal preparation from HeLa cells

Database Links

Entrez Gene ID
3852; 3856
Unigene
433845 ; 533782; 708445
SwissProt
P13647 ; P05787

Additional Information

Clone
C-50
Human Gene Symbol
KRT5 & KRT8
Chromosome Location
12q13.13 (CK5) & 17q21.2 (CK8)

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Key References

  • Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  • Vojtek et al. Folia Biol. 37(3-4): 197-206 (1991).
  • Bartek et al. J. of Pathology 164, 215-24 (1991).
  • Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).

PubMed Links

Storage & Stability

Store at 2 to 8°C. Antibody is stable for 24 months.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

0.5 ml at 200 �g/ml of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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