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Cytokeratin 5/8 Antibody [C-50]

Additional information

Catalog Number: MSM13-948
Gene:

Reactivity:

, , , , , , , ,

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM13-948-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM13-948-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM13-948-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 5/8 Antibody

SDS-PAGE Analysis of Purified Cytokeratin 5/8 Monoclonal Antibody (C-50). Confirmation of Purity and Integrity of Antibody.

Cytokeratin 5/8 Antibody - Image 2

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (C-50).

KRT5 Antibody in SDS-PAGE

KRT5 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 5 Mouse Monoclonal Antibody (C-50). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep
Clone
C-50
Isotype
IgG1
Light Chain
Kappa
Gene Name
KRT5
Positive Control
HepG2 or A431 cells. Skin or Squamous cell carcinoma.
Immunogen
Cytoskeletal preparation from HeLa cells
Storage & Stability
Store at 2 to 8°C. Antibody is stable for 24 months.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Key References
  • Bartkova et al. Neoplasma 38(4), 439-46 (1991).
  • Vojtek et al. Folia Biol. 37(3-4): 197-206 (1991).
  • Bartek et al. J. of Pathology 164, 215-24 (1991).
  • Kovaik et al. J. Tumor Marker Oncol. 5, 219 (1990).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3852; 3856
SwissProt
P13647 ; P05787
Chromosome Location
12q13.13 (CK5) & 17q21.2 (CK8)
Mol. Weight of Antigen
58kDa (CK5); 52.5kDa (CK8)
Alternate Names
Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8

Summary

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

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