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Home >> Antibodies >> Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Mouse Monoclonal Antibody [Clone KRT20/1991]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991).

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Formalin-fixed, paraffin-embedded human Normal Colon stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991).

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Western Blot Analysis of human HT29 cell lysate using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991).

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SDS-PAGE Analysis Purified Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991).

Product Details

Synonyms

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Positive Control

HT29 cells. Colon Carcinoma

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (0.5-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wauters, C.C., et al. 1995. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum. Pathol. 26: 852-855.
  2. Moll, R., et al. 1993. The human gene encoding Cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation 53: 75-93.

Bioinformatics

  •  Entrez Gene ID: 54474
  •  Gene Symbol: KRT20
  •  SwissProt: P35900
  •  Chromosome Location: 17q21.2
  •  Unigene: 84905
DOC#01991