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Home >> Antibodies >> Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Mouse Monoclonal Antibody [Clone KRT20/1991]

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Catalog No Formulation Size Price (USD)  
54474-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
54474-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
54474-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 54474
Human SwissProt P35900
Human Unigene 84905
Human Gene Symbol KRT20
Human Chromosome Location 17q21.2
Synonyms CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20
Immunogen Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 46kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others not known.

Specificity & Comments

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.

Known Applications & Suggested Dilutions

Western Blot (0.5-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HT29 cells. Colon Carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Moll, R., et al. 1993. The human gene encoding Cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation 53: 75-93.
  2. Wauters, C.C., et al. 1995. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum. Pathol. 26: 852-855.
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