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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991).
Formalin-fixed, paraffin-embedded human Normal Colon stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991).
SDS-PAGE Analysis Purified Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (KRT20/1991). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/1991).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
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