Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/3129R).

Formalin-fixed, paraffin-embedded human Colon stained with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/3129R).

SDS-PAGE Analysis Purified CK20 Recombinant Rabbit Monoclonal Antibody (KRT20/3129R). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HT29 cell lysate using CK20 Recombinant Rabbit Monoclonal Antibody (KRT20/3129R).

Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) [KRT20/3129R]

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Summary

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

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Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG
Cellular Localization
Cytoplasm
Gene Name
KRT20
Mol. Weight of Antigen
46kDa
Immunogen
Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Database Links

Entrez Gene ID
54474
Unigene
84905
SwissProt
P35900

Additional Information

Clone
KRT20/3129R
Human Gene Symbol
KRT20
Chromosome Location
17q21.2

Known Applications & Suggested Dilutions

  • Western Blot (1-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Moll, R., et al. 1993. Differentiation 53: 75-93.
  • Wauters, C.C., et al. 1995. Hum. Pathol. 26: 852-855.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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